In modern molecular biology research, Fluorescence In Situ Hybridization (FISH) has become an indispensable technique for detecting and localizing specific DNA or RNA sequences within tissue samples. Creative Bioarray offers high-quality cryosectioning services optimized for FISH analysis, providing researchers with a powerful platform for precise, sensitive, and efficient detection.

Cryosections vs. Paraffin Sections

Traditional paraffin-embedded tissue sections are widely used due to their excellent preservation of tissue morphology. However, they present challenges for FISH applications. Paraffin embedding involves high-temperature processing and strong fixation, which can degrade RNA and cause cross-linking that impedes probe hybridization.

In contrast, cryosections are prepared using milder fixation methods (typically 4% paraformaldehyde), preserving RNA integrity and enhancing probe accessibility. The preparation process is also faster and more suitable for time-sensitive or RNA-focused analyses.

DNA/RNA FISH on Cryosections

Creative Bioarray’s histology platform offers comprehensive tissue cryosectioning services for both fresh and fixed tissues. We provide cryosections with a thickness of 8–12 μm, tailored for subsequent DNA or RNA FISH analysis.

For specialized studies, we also offer LNA-FISH using Locked Nucleic Acid (LNA) probes—ideal for detecting small RNAs such as miRNAs with high sensitivity and specificity. Our team designs and customizes LNA probes to meet the specific requirements of your research project.

Key Advantages

  • High-Quality Cryosections: 8–12 μm thick sections optimized for FISH applications.
  • Custom Probe Design: Tailored LNA probe development for accurate target detection.
  • Fast Turnaround: Streamlined workflows for quicker project completion.
  • Expert Support: Professional guidance from experienced technical staff.

Creative Bioarray is committed to providing reliable, high-performance cryosection and FISH services to support your research in gene expression analysis, disease mechanisms, and more.

For more information, please visit the official website:
👉 Tissue Cryosections and FISH – Creative Bioarray